The concentration of Acute phase proteins in serum is altered as a response to inflammation, infection or trauma. this makes these proteins potential biomarkers of unspecific pathological processes. In cattle, haptoglobin and serum amyloid A are the two Acute phase proteins that show the largest increase in serum concentration as a result of inflammatory processes. The haptoglobin concentration has been shown to correlate to clinical signs as well as inflammatory processes found at post mortem examination. This feature could be useful prognosis of the individual as well as for monitoring the health status of a herd or identifying carcasses that shold be examined closer at the slaughter-house.

The analysis of acute phase protein serum amyloid A (SAA) has recently been brought into clinical use in veterinary medicine. Some of the difficulties with incorporating the SAA method in clinical practice have been the expensive and rather large equipment required for the method. Due to these difficulties only larger clinics can afford to use the SAA analysis.The company Equinostic has recently developed a smaller instrument that costs one-tenth of a larger instrument. The instrument is named EVA1 and has so far only been used to analyze SAA in horses.The aim of this study was to investigate if the EVA1 instrument could be used to analyze SAA in cats. This study included 24 serum samples from cat, which were first analyzed twice on the EVA1 instrument and then sent to the Strömsholm Referral Animal Hospital in Sweden where they reanalyzed the samples using a validated reference method.

Tumour development occurs to a large extent because of dysregulation of the cell cycle. Of particular importance are defects occurring in the G1 phase. The reason why G1 is critical is because of the influence of several signals (external signals as well as cyclins and cdks) on this stage. The G1 phase can be divided into two parts, G1pm and G1ps. Each part has its ?own? restriction point which needs to be passed in order to progress to S phase.

The veterinary handling of wildlife, both in zoos and in the wild, commonly requires that the patients need to be captured and immobilized for examination or treatment. This emphasises the need for quick, on-site, laboratory equipment, in order to minimize the analysis time and thus the stress for the animal and also in order to increase the possibility of starting relevant treatment early on. The aim of this pilot study was to determine whether Serum Amyloid A (SAA) could be a measured in blood samples from bottlenose dolphins (Tursiops truncatus), Asian elephants (Elephas maximus) and South American tapirs (Tapirus terrestris). SAA rises to its maximum about 24 hours after the introduction of an inflammatory agent in companion animals. This also correlates with the estimated time it takes for animals at Kolmården Wildlife Park to be examined and having blood samples collected after being observed as sick. The study also evaluated whether the Eurolyser SOLO, using a turbidometric immunoassay (TIA) developed for human diagnostics, could be used for such analysis. This pilot study was a retrospective analysis of SAA in convenience sampled frozen serum, collected from dolphins, elephants and tapirs.

Breeding-induced endometritis in horses seems to be down-regulated by certain components in the seminal plasma. These components are most likely some of the proteins in the seminal plasma in the stallion ejaculate, the so-called spermadhesins. This study aimed to recognize these proteins and determine their quantities in different fractions of the ejaculate. Fractionated ejaculates from four stallions were used to determine the presence and amount of proteins by way of size-excluding chromatography. All former known proteins, except two, were recognized in this study.

Laminitis is a common but devastating condition, and affected horses are often euthanized or influenced a very long time. Many horses never return to their original capacity. Laminitis is often associated with severe pain and a long time of convalescence. Symthoms includes lameness, hoof hyperthermia, strenghthened digital pulse and pain. The horse often tries to move the body weight away from the forelegs which are often more severly affected than the hindlimbs.

The cell cycle is the time a cell spends between two cell divisions. The cell cycle includes several parallel processes, all of which must be completed before a cell is mature for dividing. In the first place, all subcomponents (RNA, protein and membrane lipids) need to double in quantity and this occurs continuously through the cell cycle. Furthermore, the genome and some chromosomal proteins must double and this take place during a limited interval in the middle of the cell cycle (S-phase). On either side of the S-phase are two "gaps" which is called G1 and G2. When the cell has passed a point in the G-phase they are irreversibly programmed to progress through the remaining of the cell cycle and will undergo the next cell division. When a cell have passed a point in the G1 phase, approximately four hours after mitosis, a normal cell is in a state of indecision.

The purpose with this literature study was to investigate the possibilities for improvement of forage protein in dairy cows with focus on feeding strategies and conservation of forage protein. The utilization of forage protein in dairy cows is often not that efficient as it could be. The feed crude protein consists of both true proteins and non protein nitrogen. The digestion of proteins in the rumen depends on the protein degradation rate and the ability of the microbes to synthesize microbial protein. The microbes need a balance between carbohydrates that provide them with energy and proteins that are degradable in the rumen.

Proteins are vital to all cells in the body. They consist of long chains of amino acids. To be able to study the amino acid composition of a protein it is necessary to hydrolyse it, followed by separation and quantification. When the protein is hydrolysed, in this case ß-lactoglobulin, the protein is divided into individual amino acids. The method that traditionally has been used to hydrolyse proteins takes 24-72 hours to complete.

Bovine Respiratory Syncytial Virus (BRSV) is a common cause of respiratory disease among young cattle. The virus causes severe losses; the herd mortality rate can sometimes be as high as 20 %. In this study the avidity (i.e the antigen binding force) of BRSV specific antibodies was measured to see if there was a difference between antibodies produced during an acute phase of infection and antibodies produced by earlier infected animals. A commercially available ELISA-testkit against BRSV-specific antibodies was used and an incubation step with 6M urea was added. The effect of the urea is that it breaks the weak bonds between antibodies and antigen while the stronger bonds remain intact. Four different groups of animals were included in this study; seven calves that were naturally infected, three acutely infected calves with known time of infection, five cows that were seropositive during several years and four experimentally infected calves that had been a part of a vaccine trial. The results of this study showed that antibodies produced during the acute phase of an infection had a low avidity and that the avidity increased with time after infection.

Proteins are vital to all cells in the body. They consist of long chains of amino acids. To be able to study the amino acid composition of a protein it is necessary to hydrolyse it, followed by separation and quantification. When the protein is hydrolysed, in this case ß-lactoglobulin, the protein is divided into individual amino acids. The method that traditionally has been used to hydrolyse proteins takes 24-72 hours to complete.

Haptoglobin is an acute phase protein with important biological role because of its capacity to bind to haemoglobin. Haptoglobin exists in three major genetic polymorphism types: Hp1-1, Hp2-1 and Hp2-2, the distribution of which has been associated with abdominal aortic aneurysm (AAA), an asymptomatic aortic disease common among men older than 65 years.    Five different electrophoretic methods were tested according to their ability to separate the haptoglobin phenotypes. The detection was based on a produced hemolysate of blood in which haemoglobin binds to haptoglobin thereby forming a complex that can be detected by specific haemoglobin staining using TMB-dihydrochloride and hydro peroxide as substrate resulting in an azure-green color of the bands. Samples from 15 patients who had suffered surgery for not broken AAA, that is more than5.0 cmaortic diameter, and 15 samples from matched controls were analyzed.    Among the five tested electrophoretic methods best migration and separation was seen on the pre-cast agarosgel Hydragel HR on the instrument Hydrasys. The other four methods gave less successful results.

In this paper the authors are focusing on people that work in psychiatric acute care. How do they identify the individual that is in most need of acute care? How do they judge in this kind of cases?    The foundation in this study is in the qualitative method. The material to this study has been collected by doing qualitative interviews with six people in different professions and positions in psychiatric acute care.   The material that we collected from these six qualitative interviews has been analysed with help from Sherif?s and Hovland?s (1961) Social judgement theory.

I denna uppsats behandlas de grammatiska fenomenen complete phase och incomplete phase i det austronesiska språket rotumanska. Min avsikt med uppsatsen är att ta reda på varför complete phase respektive incomplete phase används i en viss syntaktisk eller morfologisk kontext. Jag har baserat denna studie endast på litteratur, främst från C.M Churchwards Rotuman Grammar and Dictionary..

In couples diagnosed with unexplained infertility, the woman has normal ovulatory cycles and no organic pathology, and the man has normal semen. The period during which the implantation of an embryo can occur is defined as the window of implantation (WOI). For infertile women, disturbances in the endometrium maturation are hypothesized to impair the uterine receptivity for embryo implantaition. However, there are still no methods found to predict endometrial receptivity.Hemoglobin related-proteins hypothesized to be involved in embryo implantation have been found in the endometrium in both fertile and infertile women. The aim of this study was to determine whether there were differences in the expression of hemoglobin-related proteins, hemoglobin-?, cytoglobin, fetal hemoglobin and haptoglobin in the endometrium of fertile and infertile women.